Chemical constituent, antibacterial and antioxidant activity of crude extract and oil fraction of L. Abyssinica

Publication Type

Journal Article

Journal Name

International Journal of Phytomedicine

Publication Date

1-1-2014

Abstract

Theantibacterial and antioxidant activitiesof CH3Cl/MeOH crude extract and a nonpolar fraction from the CHCl3 phase of Ludwigaa abyssnnicawere investigated using broth dilution method and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging assay respectively. Chemical composition was determined by gas chromatography coupled with mass spectrometry using Agilent Technologies 7890A GC system-Agilent Technologies 5975C inert XL EI/CI MSD. Bioassays show that L. abyssinicais active on the different test organisms with the MIC ranging from 0.5 to 1.5 mg/ml for the crude extract and 0.2 to 0.5 mg/ml for the non polar oil fraction. The oil extract of L. abyssinica exhibited a good antioxidant activity with radical scavenging activity (IC50) = 89.18 µg/ml which is about 1.5 times the antioxidant activity of ascorbic acid used as control,while that of crude extract was 413.74 µg/ml. From the gas chromatography coupled with mass spectrometry analyses, 12 hydrocarbons were identifiedwith octadecane (5.67%), pentadecane(6.87%) andsqualene (57.18%) the major components.These results offer a platform of using L. abyssinicafor alternative and complementary medicine.

Keywords

Antibacterial, Antioxidant, Column chromatography, GC-MS, Ludwigia abyssinica

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