Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus

Publication Type

Journal Article

Journal Name

Current Microbiology

Name of Author

Edward O. Omolo, International Centre of Insect Physiology and Ecology Nairobi
M. Diane James, University of Cape Town
Ellie O. Osir, International Centre of Insect Physiology and Ecology Nairobi
Jennifer A. Thomson, University of Cape Town

Publication Date

2-1-1997

Abstract

A local isolate of Bacillus thuringiensis, designated L1-2, that is toxic to Chilo partellus was found to be toxic to the adult tsetse fly, Glossina morsitans morsitans. The δ-endotoxin crystals derived from the isolate gave a major protein band with a molecular weight of M(r) 130,000- 140,000 on denaturing polyacrylamide gel electrophoresis. The sequence of the cloned gene was found to be similar to that of the B. thuringiensis subsp. kurstaki HD-73 cryIA(c) gene, having one amino acid difference at position 148 and four additional DNA differences.

PubMed ID

9003589

Recommended Citation

Omolo, E., James, M., Osir, E., & Thomson, J. (1997). Cloning and expression of a Bacillus thuringiensis (L1-2) gene encoding a crystal protein active against Glossina morsitans morsitans and Chilo partellus. Current Microbiology, 34 (2), 118-121. https://doi.org/10.1007/s002849900154