Detection of trypanosome infections in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle

Publication Type

Journal Article

Journal Name

Parasitology

Name of Author

P. A.O. Majiwa, International Laboratory for Research on Animal Diseases, Kenya
R. Thatthi, International Laboratory for Research on Animal Diseases, Kenya
S. K. Moloo, International Laboratory for Research on Animal Diseases, Kenya
J. H.P. Nyeko, International Laboratory for Research on Animal Diseases, Kenya

Publication Date

1-1-1994

Abstract

Relatively simple protocols employing non-radioactive DNA probes have been used for the detection of African trypano-somes in the blood of mammalian hosts and the saliva of live tsetse flies. In combination with the polymerase chain reaction (PCR), the protocols revealed trypanosomes in buffy-coat samples from antigenaemic but aparasitaemic cattle and in the saliva of live, infected tsetse flies. Furthermore, the protocols were used to demonstrate concurrent natural infections of single tsetse flies with different species of African trypanosomes. © 1994, Cambridge University Press. All rights reserved.

Keywords

digoxigenin, polymerase chain reaction, trypanosomes, tsetse vector

PubMed ID

8022657

Recommended Citation

Majiwa, P., Thatthi, R., Moloo, S., & Nyeko, J. (1994). Detection of trypanosome infections in the saliva of tsetse flies and buffy-coat samples from antigenaemic but aparasitaemic cattle. Parasitology, 108 (3), 313-322. https://doi.org/10.1017/S0031182000076150