Publication Type

Journal Article

Journal Name

Comparative Biochemistry and Physiology -- Part B: Biochemistry and

Publication Date

1-1-1991

Abstract

1. 1. The larval haemolymph of the stem-borer, Busseola fusca Fuller (Lepidoptera: Noctuidae) contains a high molecular weight protein (Mr ∼ 500,000) that is induced only during diapause. This diapause-induced protein, termed Busseola diapause protein (BDP), has been previously isolated and characterized. 2. 2. In this study, BDP has been shown to be a specific juvenile hormone (JH) binder by labelling with epoxybisfarnesyl diazoacetate (EBDA), the photoactivatable analog of JH I (10R, 11S). 3. 3. In a direct binding assay, BDP was also shown to bind [3H]-JH I (10R, 11S) with high affinity (Kd ∼ 120 nM). 4. 4. These results suggest that BDP might play an important role during diapause by binding and transporting JH within the haemolymph. © 1991.

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