Biochemical and immunochemical evidence for the origin of the spermatophore material in Glossina morsitans morsitans Westwood

Publication Type

Journal Article

Journal Name

Experientia

Publication Date

4-1-1987

Abstract

Protein patterns in secretions from fully differentiated male accessory reproductive glands (ARG), spermatophore (Sp) and testes (Te) of the tsetse, Glossina morsitans morsitans Westwood, were determined by isoelectrofocusing. Isoelectrofocusing patterns of total ARG proteins and those of Sp were remarkably similar. At least 27 bands were detected in ARG and Sp. Out of these, 13 were major protein bands and isoelectrofocused in the pl range of 4 and 6.55. About 10 of these 13 were found to be acidic. Ouchterlony immunodiffusion and straight line immunoelectrophoresis showed that male accessory reproductive gland secretory proteins and spermatophore share common immunological characteristics which are different from those of the testes. © 1987 Birkhäuser Verlag.

Keywords

double immunodiffusion, Glossina morsitans, immunoelectrophoresis, isoelectrofocusing, male accessory reproductive glands, protein patterns, spermatophore, testes

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