Spatio-temporal distribution of sugarcane shoot borer, Chilo infuscatellus (Lepidoptera: Crambidae) and its associated natural enemy Sturmiopsis inferens (Diptera:Tachinidae) in India

Publication Type

Journal Article

Journal Name

European Journal of Horticultural Science

Publication Date

1-1-2016

Abstract

Background of the study: Food and nutritional security remain a great challenge especially in developing countries. To address this challenge the exploration of natural resources such as mushrooms should be considered. Mushrooms species can be morphologically similar but biochemically different, hence the need for accurate identification. Objective: The objective of this study was to identify indigenous mushrooms in Niger Delta region of Nigeria using molecular tools (ITS barcoding). Methods: Mushroom samples (N=57) were collected across six states of the Niger Delta Region: Abia, Akwa Ibom, Bayelsa, Delta, Imo and Rivers. ITS DNA barcoding was used for species identification. The ITS1, 5.8S rDNA and ITS2 locus was amplified. Results: Nucleotide sequences analysis revealed genetic diversity among samples with 57 isolates belonging to 27 species of 14 different families identified. The family Polyporaceae was the most abundant comprising five species, 23 isolates with 40.4%, followed by Pleurotaceae comprising five species, ten isolates with 17.5 %. Conclusion: Indigenous mushrooms in The Niger Delta Region of Nigeria are diverse. ITS barcoding was effective in identification of samples. This study will promote knowledge of the diverse species. As a result, it will enhance cultivation and consumption of indigenous wild mushrooms and also will improve livelihoods for small holder farmers.

Keywords

Barcoding, Biodiversity, Indigenous wild mushroom, ITS, Niger Delta Region, Species identification

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