Trypanosoma Congolense Resistant to Trypanocidal Drugs Homidium and Diminazene and their Molecular Characterization in Lambwe, Kenya

Publication Type

Journal Article

Publication Date (Issue Year)

2023

Journal Name

Acta Parasitologica

Abstract

Purpose

African animal trypanosomiasis (AAT) is a disease affecting livestock in sub-Saharan Africa. The use of trypanocidal agents is common practice to control AAT. This study aimed to identify drug-resistant Trypanosoma congolense in Lambwe, Kenya, and assess if molecular test backed with mice tests is reliable in detecting drug sensitivity.

Methods

Blood samples were collected from cattle, in Lambwe, subjected to buffy coat extraction and Trypanosoma spp. detected under a microscope. Field and archived isolates were subjected to molecular characterization. Species-specific T. congolense and TcoAde2 genes were amplified using PCR to detect polymorphisms. Phylogenetic analysis were performed. Four T. congolense isolates were evaluated individually in 24 test mice per isolate. Test mice were then grouped (n=6) per treatement with diminazene, homidium, isometamidium, and controls. Mice were subsequently assessed for packed cell volume (PCV) and relapses using microscopy.

Keywords

Keywords

Rsif Scholar Name

Ivy Sylvia Okello

Rsif Scholar Nationality

Kenya

Cohort

Cohort 2

Thematic Area

Food security and Agribusiness

Africa Host University (AHU)

Sokoine University of Agriculture (SUA), Tanzania

Funding Statement

This study was funded by Partnership for Skills in Applied Sciences, Engineering and Technology-Regional Scholarship Innovative Fund. The funders had no role in developing the study design, collection, analysis, interpretation of the results, and writing the manuscript.

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