Publication Type
Journal Article
Publication Date (Issue Year)
2024
Journal Name
Tropical Medicine and Infectious Disease
Abstract
The resistance of trypanosomes to the doses of trypanocide administered by farmers to theiranimals acts as a real brake on efforts to control to combat African trypanosomiasis. Thus, in-depthknowledge of the use of these different molecules and their resistance profiles will be necessary toestablish an integrated strategy to combat African trypanosomiasis. To achieve these objectives, aparticipatory survey among farmers and a resistance diagnosis of trypanosome strains identifiedin three regions of northern Côte d’Ivoire (Bagoué, Poro and Tchologo) was carried out using thePCR-RFLP technique, followed by sequencing of genes of interest. This study made it possible toidentify three molecules that are commonly used by 85% (63/74) of farmers. In descending orderof use, we identified Isometamidium chloride (43%), Diminazene aceturate (28%) and Homidiumbromide (14%). Three species of trypanosomes,Trypanosoma congolense,Trypanosoma. theileriandTrypanosoma vivax, were identified in farms, and only one strain had the adenosine transportergene (Trypanosoma congolense), but this strain was sensitive to the Diminazene aceturate molecule.Comparison of the sequence of this trypanosome strain showed that it is different to the Kenyanstrain diagnosed as resistant to the Diminazene aceturate molecule. This study shows that a varietyof trypanocides are used by farmers, and that the resistance profile of the strains to the Diminazeneaceturate molecule could not be observed. However, it is important to further investigate the othermolecules encountered in Côte d’Ivoire
Keywords
trypanocide; resistance; trypanosome; cattle; Côte d’Ivoire
Rsif Scholar Name
Jean-Yves Ekra
Thematic Area
Food security and Agribusiness
Africa Host University (AHU)
Sokoine University of Agriculture (SUA), Tanzania
Funding Statement
This research was funded by the Partnership for Skills in Applied Sciences, Engineering,and Technology (PASET) through the Regional Scholarship and Innovation Fund (RSIF), whichawarded Jean-Yves EKRA a doctoral scholarship at the SACIDS Africa Centre of Excellence for Infec-tious Diseases, SACIDS Foundation for One Health, Sokoine University of Agriculture, Morogoro,Tanzania. The APC was financed by PASET-RSIF. The funders had no input into the study’s design,data collection and analysis, publishing decision or manuscript writing. The study’s findings andconclusions are those of the authors and do not necessarily represent the views of the sponsors.
Recommended Citation
Ekra, J., Mafie, E. M., Sonan, H., Kanh, M., Gragnon, B. G., N’Goran, E. K., & Srinivasan, J. (2024). Trypanocide Use and Molecular Characterization of Trypanosomes Resistant to Diminazene Aceturate in Cattle in Northern Côte D’Ivoire. Tropical Medicine and Infectious Disease, 9 (9), 192. https://doi.org/10.3390/tropicalmed9090192