Publication Type
Journal Article
Journal Name
Scientific Reports
Publication Date
12-1-2024
Abstract
Phthorimaeaabsoluta is an invasive solanaceous plant pest with highly devastating effects on tomato plant. Heavy reliance on insecticide use to tackle the pest has been linked to insecticide resistance selection in P.absoluta populations. To underline insights on P.absoluta insecticide resistance mechanisms to diamides and avermectins, we evaluated the transcriptomic profile of parental (field-collected) and F8 (lab-reared) populations. Furthermore, to screen for the presence of organophosphate and pyrethroid resistance, we assessed the gene expression levels of acetylcholinesterase (ace1) and para-type voltage-gated sodium channel (VGSG) genes in the F1 to F8 lab-reared progeny of diamide and avermectin exposed P.absoluta field-collected populations. The VGSG gene showed up-regulation in 12.5% and down-regulation in 87.5% of the screened populations, while ace1 gene showed up-regulation in 37.5% and down-regulation in 62.5% of the screened populations. Gene ontology of the differentially expressed genes from both parental and eighth generations of diamide-sprayed P.absoluta populations revealed three genes involved in the metabolic detoxification of diamides in P.absoluta. Therefore, our study showed that the detoxification enzymes found could be responsible for P.absoluta diamide-based resistance, while behavioural resistance, which is stimulus-dependent, could be attributed to P.absoluta avermectin resistance.
PubMed ID
38575641
Funding Statement
The authors gratefully acknowledge the financial support for this research by the following organizations and agencies: The African Union (AU) (Tuta-IPM Project, Contract number: AURG II-2-123-2018) and UK’s Foreign, Commonwealth & Development Office (FCDO) (FCDO Biopesticide Project, B2291A-FCDO-BIOPESTICIDE); the Swedish International Development Cooperation Agency (Sida); the Swiss Agency for Development and Cooperation (SDC); the Australian Centre for International Agricultural Research (ACIAR); the Norwegian Agency for Development Cooperation (Norad); the German Federal Ministry for Economic Cooperation and Development (BMZ); and the Government of the Republic of Kenya. The views expressed herein do not necessarily reflect the official opinion of the donors. S.W.K. was supported by AU-Tuta-IPM Project through the Dissertation and Research Internship Program (DRIP) of icipe. We gratefully acknowledge Mr Fidelis L. Ombura and Mr Sospeter Wafula for their technical assistance.
Recommended Citation
Karanu, S., Ajene, I., Lelmen, E., Ong’onge, M., Akutse, K., & Khamis, F. (2024). Biochemistry and transcriptomic analyses of Phthorimaeaabsoluta (Lepidoptera: Gelechiidae) response to insecticides. Scientific Reports, 14 (1) https://doi.org/10.1038/s41598-024-58413-z