Publication Type

Journal Article

Publication Date (Issue Year)

2022

Journal Name

All Life

Abstract

Maize Lethal Necrosis (MLN) is a disease of maize reported in Kenya in 2012 that results in yield lossesof up to 100%. The epidemiology of MLN is complex as the disease is caused by the synergisticinteraction of 2 viruses (Maize chlorotic mottle virus(MCMV) and a potyvirus). In addition, multiplereservoirs and transmission pathways exist for the spread of MLN. The current study was conductedto understand farmers’ maize production practices, their understanding of MLN, and the status ofMLN in Kenya. Therefore, a survey of 406 randomly selected farmers was conducted in Bomet, Narok,Kirinyaga, Embu, and Nakuru. To confirm the presence of MLN, maize leaf samples were collectedfrom 18 fields and tested for MCMV and SCMV by molecular techniques. MLN Symptoms observedincluded chlorotic mottle on leaves, necrosis, and premature plant death. MCMV and SCMV weredetected in all the maize growing regions at varying levels of incidence, and severity. Sequenceanalysis of the partial coat protein genes of randomly selected positive samples of the two virusesshowed little variability within the studied isolates and those retrieved from the GenBank. The resultsindicated that MLN is still prevalent in Kenya with farmers’ planting susceptible varieties.

Keywords

farmers’ perceptionand, maize, lethal necrosis, Kenya

Rsif Scholar Name

Faith Njeri Njeru

Rsif Scholar Nationality

Kenya

Cohort

Cohort 2

Thematic Area

Food security and Agribusiness

Africa Host University (AHU)

Sokoine University of Agriculture (SUA), Tanzania

Funding Statement

This manuscript is part of a Ph.D. project (Application ofNanobody as a diagnostic tool against Maize Lethal Necrosis)funded by the Partnership for Skills in Applied Sciences, Engi-neering and Technology (PASET), Regional Scholarship andInnovation Fund, (RSIF).

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