Publication Type
Journal Article
Publication Date (Issue Year)
2020
Journal Name
Antibiotics
Abstract
Campylobacter species have developed resistance to existing antibiotics. The development of alternative therapies is, therefore, a necessity. This study evaluates the susceptibility of Campylobacter strains to selected natural products (NPs) and frontline antibiotics. Two C. jejuni strains (ATCC® 33560TM and MT947450) and two C. coli strains (ATCC® 33559TM and MT947451) were used. The antimicrobial potential of the NPs, including plant extracts, essential oils, and pure phytochemicals, was evaluated by broth microdilution. The growth was measured by spectrophotometry and iodonitrotetrazolium chloride. Antibiotic resistance genes (tet(O) and gyrA) were characterized at the molecular level. The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) ranged from 25 to 1600 µg/mL. Cinnamon oil, (E)-Cinnamaldehyde, clove oil, eugenol, and baicalein had the lowest MIC and MBC values (25–100 µg/mL). MT947450 and MT947451 were sensitive to erythromycin and gentamicin but resistant to quinolones and tetracycline. Mutations in gyrA and tet(O) genes from resistant strains were confirmed by sequencing. The findings show that NPs are effective against drug-sensitive and drug-resistant Campylobacter strains. The resistance to antibiotics was confirmed at phenotypic and genotypic levels. This merits further studies to decipher the action mechanisms and synergistic activities of NPs.
Keywords
antibiotics, Campylobacter, plant extracts, essential oils, phytochemicals, resistance
Rsif Scholar Name
Gahamanyi Noel
Thematic Area
Food security and Agribusiness
Africa Host University (AHU)
Sokoine University of Agriculture (SUA), Tanzania
Funding Statement
This work was financially supported by the Ministry of Oceans and Fisheries, Korea (grant number 20170488), and the Partnership for Skills in Applied Sciences, Engineering, and Technology—Regional Scholarship and Innovation Fund (PASET-RSIF) in collaboration with the Government of the Republic of Korea. Natural product extracts were provided by the KIST Natural Product Library, supported by KIST institutional program (2Z05640), and the article processing charge (APC) was funded by KIST.
Recommended Citation
Noel, G. (2020). Susceptibility of campylobacter strains to selected natural products and frontline antibiotics. Antibiotics, 9 (11), 1-14. https://doi.org/10.3390/antibiotics9110790